| | Organization Name(s) | Located In |
 | ABC Day Nursery of Windsor, Lauzon Road | Windsor East |
 | ABC Day Nursery of Windsor, Somme St | Windsor East |
 | Audrey's Wee Horizons Childcare | Leamington |
 | Building Healthy Babies Program, Chatham-Kent Public Health Unit | Chatham |
 | Can-Am Indian Friendship Centre of Windsor Inc, Urban Aborignal Healthy Living Program | Windsor East |
 | Chatham-Kent Breastfeeding Coalition, Chatham-Kent Public Health Unit | Chatham |
 | Chez Nous Child Care Inc | Windsor East |
 | Children First | Windsor East |
 | Children's Hospital London Health Sciences Centre | London |
 | Children's Hospital London Health Sciences Centre, Child and Adolescent Mental Health Care Program | London |
 | Children's Hospital London Health Sciences Centre, Paediatric Diabetes Team | London |
 | Children's Hospital London Health Sciences Centre, Pediatric Symptom Management and Supportive Care | London |
 | Children's House Montessori (The) | Windsor South |
 | First Steps Child Care Centre, Tecumseh Site | Tecumseh (Town of) |
 | Franco-Sol Garderie et centre de ressources, École Monseigneur Augustin Caron | LaSalle |
 | Franco-Sol Garderie et centre de ressources, École St Edmond | Windsor South |
 | Franco-Sol Garderie et centre de ressources, École Ste Marguerite d'Youville | Tecumseh |
 | Generations Day Care Inc | Petrolia |
 | Great Beginnings Child Centered Co-operative Inc, Day Nursery | Windsor West |
 | Lakeview Montessori School | Tecumseh |
| | LeeLee Hats | Windsor |
| | Lice Squad Canada Inc, The Nitpickers | |
 | Middlesex-London Health Unit, Family Health Services | London |
 | Middlesex-London Health Unit, Family Health Services, Blind Low Vision Early Intervention Program | London |
 | Middlesex-London Health Unit, Family Health Services, Community Developmental Screening Program | London |
 | Middlesex-London Health Unit, Family Health Services, Growth & Development - Is Your Baby Progressing | Strathroy |
 | Middlesex-London Health Unit, Family Health Services, Infant Hearing Program - Southwest Region | London |
 | Middlesex-London Health Unit, Family Health Services, InfantLine | London |
 | Middlesex-London Health Unit, Family Health Services, Let's Grow Free Parenting E-Newsletter | London |
 | Miss Helen's Place | Sarnia |
 | Once Upon A Time Child Care Centre, Cottam | Cottam |
 | Once Upon A Time Child Care Centre, Essex | Essex (Town of) |
 | Once Upon A Time Child Care Centre, Maidstone | Maidstone (Village of) |
 | Ready Set Go! | Windsor Central |
 | Ready Set Go!, Holiday Programs | Windsor Central |
 | Ready Set Go!, Preschool Family Drop-in Centre | Windsor Central |
 | Salvation Army (The), Eastwood Citadel Community Church, Learning Corner Child Care Centre | Windsor East |
 | Sonshine Day Care Centre of Belle River | Belle River (Town of) |
 | Sonshine Day Care Centre of Belle River, Latch Key Program | Belle River (Town of) |
 | Story Book Early Learning Centre | Essex (Town of) |
 | Story Book Early Learning Centre, Essex Public School Site | Essex (Town of) |
 | Tilbury Tots Early Learning Centre, Main Site - Tilbury | Tilbury |
 | Tilbury Tots Early Learning Centre, T.T.E.L.C. at École Ste-Marie | Chatham |
 | Tilbury Tots Early Learning Centre, T.T.E.L.C. at École St-Francis | Tilbury East |
 | Tilbury Tots Early Learning Centre, T.T.E.L.C. at École St-Paul | Stoney Point |
 | Tilbury Tots Early Learning Centre, T.T.E.L.C. at Merlin Area Public School | Merlin |
 | Tilbury Tots Early Learning Centre, T.T.E.L.C. at Pain Court Hub, Sainte-Catherine Garderie | Pain Court |
 | Tilbury Tots Early Learning Centre, T.T.E.L.C. at St. Francis Catholic School | Tilbury |
| | Tilbury Tots Early Learning Centre, T.T.E.L.C. at Tilbury Area Public School | Tilbury |
 | Tilbury Tots Early Learning Centre, T.T.E.L.C. at Wheatley Area Public School | Wheatley |
 | Toy Box Inc (The), Early Childhood Education Centre, Howard Ave Site | Windsor South |
 | Toy Box Inc (The), Early Childhood Education Centre, McDougal Site | Windsor South |
 | Windsor-Essex County Health Unit, Clinical Services | Windsor Central |
 | Windsor Regional Hospital, Metropolitan Campus, Rehabilitation Services, Infant Hearing Screening Program | Windsor Central |
 | Your Wooden Tree House, Child Care Centre, Emeryville | Emeryville |