| | Organization Name(s) | Located In |
 | A Place to Grow, St Christopher Site | Windsor Central |
| A Smart Start Day Care | Tecumseh (Town of) |
| Aamjiwnaang Day Nursery | Sarnia |
| ABC Child Care Centre | Sarnia |
| ABC Day Nursery of Windsor, Arthur | Windsor East |
| ABC Day Nursery of Windsor, Hanna St | Windsor Central |
| ABC Day Nursery of Windsor, Lauzon Road | Windsor East |
| ABC Day Nursery of Windsor, Somme St | Windsor East |
| Aboriginal Child Resource Centre | Windsor Central |
| Acorn Christian Day Care | London |
| Adventure Hours Nursery School, Chatham | Chatham |
| Alexander's Day Care Center | Windsor East |
| Arbour Glen Day Nursery | London |
| Arva's Little School House | London |
| Audrey's Playground Daycare | Leamington |
| Audrey's Wee Horizons Childcare | Leamington |
| Bayshore Home Health | Windsor Central |
| Beacons Of Light Daycare | Sarnia |
| Blossoms Early Childhood Education Centre | London |
| Bright Beginnings Early Childhood Centre | London |
| Brooke-Alvinston Child Care Centre | Alvinston |
| | Brooke-Alvinston Children's Co-Operative Inc. | Alvinston |
| Byejwanong Children's Centre, Wallaceburg | Wallaceburg |
| Can-Am Indian Friendship Centre of Windsor Inc, Aboriginal Healthy Babies Healthy Children | Windsor East |
| Chatham-Kent Breastfeeding Coalition, Chatham-Kent Public Health Unit | Chatham |
| Chatham-Kent Family YMCA, Our Little Place Child Development Centre | Chatham |
| Chatham-Kent May Court Childcare Learning Centre, Chatham | Chatham |
| Chatham-Kent Wallaceburg Childcare Learning Centre, Municipality of Chatham-Kent | Wallaceburg |
| Chelsea Green Children's Centre | London |
| Chez Nous Child Care Inc | Windsor East |
| Children's House Montessori (The) | Windsor South |
| Children's House Montessori (The), LaSalle | LaSalle |
| Children's Place Child Care Centre | London |
| Children's Village Daycare Centre, Chatham | Chatham |
| Christian Kid's Club Nursery School | Dorchester |
| Connections Early Years Family Centre, Be Back Soon Program | Windsor Central |
| Coronation Park Day Nursery | Sarnia |
| Cronyn Child Care Centre | London |
| Delta Chi Cosmopolitan Kids, Hospital Campus | Windsor Central |
| Discovery School Based Childcare Program, Kingsville, Childcare and Latchkey Program, St John de Brebeuf Elementary School | Kingsville |
| Discovery School Based Childcare Program, Kingsville, Childcare Program, Kingsville Elementary School | Kingsville |
| Discovery School Based Childcare Program, Kingsville, Childcare/Latchkey Program, Jack Miner Elementary School | Kingsville |
| Discovery School Based Childcare Program, Kingsville, Early Years Enrichment Program, Kingsville Branch | Kingsville |
| Discovery School Based Childcare Program, Kingsville, Latchkey Program, Queen Elizabeth Elementary School | Leamington |
| Dorchester Cooperative Nursery School | Dorchester |
| École Élémentaire L'Envolée, La Garderie Les Petites Mains Windsor Inc | Windsor Central |
| Faith Day Nursery | London |
| Family Centre (The) | Wallaceburg |
| First Steps Child Care Centre, Tecumseh Site | Tecumseh (Town of) |
| Franco-Sol Garderie et centre de ressources, École George P. Vanier | Windsor East |
| Franco-Sol Garderie et centre de ressources, École Mgr Jean Noël | Windsor West |
| Franco-Sol Garderie et centre de ressources, École Monseigneur Augustin Caron | LaSalle |
| Franco-Sol Garderie et centre de ressources, École Pavillon des jeunes | Emeryville |
| Franco-Sol Garderie et centre de ressources, École St Antoine | Tecumseh |
| Franco-Sol Garderie et centre de ressources, École St Jean Baptiste | Amherstburg |
| Franco-Sol Garderie et centre de ressources, École St Michel | Leamington (Town of) |
| Franco-Sol Garderie et centre de ressources, École Ste Marguerite d'Youville | Tecumseh |
| Franco-Sol Garderie et centre de ressources, École Ste Thérèse | Windsor East |
| Generations Day Care Inc | Petrolia |
| Grand Avenue Children's Centre | London |
| Great Beginnings Child Centered Co-operative Inc | Windsor West |
| Great Beginnings Child Centered Co-operative Inc, Day Nursery | Windsor West |
| Growing Concern Child Care Centre | London |
| Growing Together, Growing Together at St Annes, Growing Together at Harwich Raleigh, Growing Together at WJ Baird | Blenheim |
| Growing Together Family Resource Center | Blenheim |
| | Home Childcare Providers' Network | Windsor |
| Jack N'Jill Nursery School, Ridgetown | Ridgetown |
| John McGivney Children's Centre | Windsor West |
| John McGivney Children's Centre, Summer Programs | Windsor West |
| Kangaroo's Pouch Day Care | London |
| KidLogic Child Care Learning Centre | London |
| Kids' Ark Day Nursery | London |
| Kid's Stuff... the Family Learning Centre on the Thames, Chatham, Gregory Drive School Campus | Chatham |
| Kid's Stuff... the Family Learning Centre on the Thames, Chatham, Queen Elizabeth II Public School location | Chatham |
| Kid's Stuff... the Family Learning Centre on the Thames, Chatham, St Joseph's Separate School location | Chatham |
| Kid's Stuff... the Family Learning Centre on the Thames, Chatham, Thames Campus | Chatham |
| Kid's Stuff... the Family Learning Centre on the Thames, Chatham, Winston Churchill School Campus | Chatham |
| KidZone Day Care Centre | London |
| Kilworth Children's Centre | Komoka |
| Lakeshore Township Community Daycare Centre | Lakeshore |
| Lakeview Montessori School | Tecumseh |
| Lambton Rural Child Care | Petrolia |
| Latchkey Day Care & Learning Centre, Administration Office | Tecumseh (Town of) |
| Latchkey Day Care & Learning Centre, Notre Dame Latchkey Program | Windsor West |
| Latchkey Day Care & Learning Centre, Our Lady of Mount Carmel Latchkey Program | Windsor South |
| Latchkey Day Care & Learning Centre, Our Lady of the Annunciation Latchkey Program | Stoney Point |
| Latchkey Day Care & Learning Centre, Prince Andrew Latchkey Program | LaSalle |
| Latchkey Day Care & Learning Centre, Sacred Heart Latchkey Program | LaSalle |
| Latchkey Day Care & Learning Centre, Sandwich West Latchkey Program | LaSalle |
| Latchkey Day Care & Learning Centre, St Gregory Latchkey Program | Lakeshore |
| Latchkey Day Care & Learning Centre, St John the Baptist Latchkey Program | Belle River (Town of) |
| Latchkey Day Care & Learning Centre, St Joseph Latchkey Program | River Canard |
| Latchkey Day Care & Learning Centre, St Peter Latchkey Program | Tecumseh (Town of) |
| Latchkey Day Care & Learning Centre, St Pius X Latchkey Program | Tecumseh (Town of) |
| Latchkey Day Care & Learning Centre, Victoria Latchkey Program | Tecumseh |
| Learning Circle Preschool | Petrolia |
| Little Friends Child and Family Development Centre, Ska:na Family Learning Centre | Sarnia |
| Little Lambs Day Care | Strathroy |
| Little Red Schoolhouse (The) | London |
| London Bridge Child Care Services | London |
| London Bridge Child Care Services, Adelaide Early Childhood Learning Centre | London |
| London Bridge Child Care Services, Elmwood Early Childhood Learning Centre | London |
| London Bridge Child Care Services, Huron Heights Early Childhood Learning Centre | London |
| London Bridge Child Care Services, Little Acorns Early Childhood Learning Centre | London |
| London Bridge Child Care Services, London Day Nursery Early Childhood Learning Centre | London |
| London Bridge Child Care Services, Maitland Early Childhood Learning Centre | London |
| London Bridge Child Care Services, Piccadilly Place Early Childhood Learning Centre | London |
| London Bridge Child Care Services, Rowntree Park Early Childhood Learning Centre | London |
| London Bridge Child Care Services, Springbank Early Childhood Learning Centre | London |
| London Bridge Child Care Services, Stoneybrook Early Childhood Learning Centre | London |
| London Children's Connection | London |
| London Children's Connection, Ashley Oaks Children's Centre | London |
| London Children's Connection, Bonaventure Meadows Children's Centre | London |
| London Children's Connection, Byron Somerset Children's Centre | London |
| London Children's Connection, Community Home Child Care | London |
| London Children's Connection, Jack Chambers Children's Centre | London |
| London Children's Connection, Jean Vanier Children's Centre | London |
| London Children's Connection, North London Children's Centre | London |
| London Children's Connection, Pond Mills Children's Centre | London |
| London Children's Connection, Rick Hansen Children's Centre | London |
| London Children's Connection, Westminster Children's Centre | London |
| London Children's Connection, White Oaks Children's Centre | London |
| Miss Helen's Place | Sarnia |
| Montessori School of Windsor, Grand Marais | Windsor South |
| Montessori School of Windsor, Labelle St | Windsor South |
| Moravian Day Care Centre, Thamesville | Thamesville |
| Mulberry Bush Child Centre | London |
| | Neighbourlink Sarnia-Lambton | Sarnia |
| North Lambton Childcare Centre - Forest | Forest |
| Oak Park Co-operative Children's Centre | London |
| Once Upon A Time Child Care Centre, Cottam | Cottam |
| Once Upon A Time Child Care Centre, Essex | Essex (Town of) |
| Once Upon A Time Child Care Centre, Maidstone | Maidstone (Village of) |
| Ontario Early Years Centre, Arkona Outreach | Arkona |
| Ontario Early Years Centre, Franco-Sol Garderie et centre de ressources, Resource Centre and Toy Lending library, Satellite Centre | Windsor West |
| Ontario Early Years Centre, Sarnia Lambton | Sarnia |
| Ontario Early Years Centre, Ska:na Family Learning Centre | Windsor East |
| Ontario Early Years Centre Alvinston Site | Alvinston |
| Ontario Early Years Centre Camlachie Outreach | Camlachie |
| Ontario Early Years Centre Forest Site | Forest |
| Ontario Early Years Centre Grand Bend Outreach | Grand Bend |
| Ontario Early Years Centre Outreach, Brigden | Brigden |
| Ontario Early Years Centre Petrolia Site | Petrolia |
| Ontario Early Years Centre Thedford Outreach | Thedford |
| Ontario Early Years Centre Watford Outreach | Watford |
| Ontario Early Years Centre Wyoming Outreach | Wyoming |
| Ontario. Ministry of Community and Social Services - South West Region, Ontario Disability Support Program (ODSP) - London | London |
| Orchard Park Nursery School | London |
| Our Kids Child Care | Sarnia |
| Outreach Program | Sarnia |
| Oxford Community Child Care, Garderie francophone du comté d'Oxford | Woodstock |
| | Oxford County. Public Health and Emergency Services, Public Health, Oxford Child Care Guide | Woodstock |
| Point Edward Early Learning Centre | Point Edward |
| Ribambelle centre préscolaire Francophone de London, Academie de la Tamise | London |
| Ribambelle centre préscolaire Francophone de London, Frère-André | London |
| Ribambelle centre préscolaire Francophone de London, Marie-Curie | London |
| Ribambelle centre préscolaire Francophone de London, Ridgewood | London |
| Ribambelle centre préscolaire Francophone de London, Saint-Jean-de-Brébeuf | London |
| Ridge KIDS, Kids Incorporated Daycare Services, Ridgetown | Ridgetown |
| Salvation Army (The), Eastwood Citadel Community Church, Learning Corner Child Care Centre | Windsor East |
| Salvation Army (The) - London, London Village Day Nursery | London |
| Sarnia Christian School | Sarnia |
| Simply Kids - Neighbourhood Child Care and Learning Centre | London |
| Ska:na Family Learning Centre, Ontario Early Years Centre, Aboriginal Site | Windsor East |
| Small World Nursery School | Thedford |
| Sombra Township Child Care | Sombra |
| Sonshine Day Care Centre of Belle River | Belle River (Town of) |
| Southdale Day Care Centre | London |
| St Joseph's Health Care, London, Parkwood Hospital, Parkwood Children's Day Care Centre | London |
| Story Book Early Learning Centre | Essex (Town of) |
| Story Book Early Learning Centre, Essex Public School Site | Essex (Town of) |
| Strathroy Community Christian School | Strathroy |
| Sundowners Day Care and Resource Centre | Windsor Central |
| Sundowners Day Care and Resource Centre, Christ the King School Child Care Centre | Windsor South |
| Sundowners Day Care and Resource Centre, Dougall School Child Care Centre | Windsor Central |
| Sundowners Day Care and Resource Centre, Forest Glade Primary Learning Centre Child Care Centre | Windsor East |
| Sundowners Day Care and Resource Centre, General Brock School Child Care Centre | Windsor West |
| Sundowners Day Care and Resource Centre, Glenwood School Child Care Centre | Windsor South |
| Sundowners Day Care and Resource Centre, Gordon McGregor School Child Care Centre | Windsor East |
| Sundowners Day Care and Resource Centre, Holy Cross School Child Care Centre | LaSalle |
| Sundowners Day Care and Resource Centre, Hugh Beaton School Child Care Centre | Windsor Central |
| Sundowners Day Care and Resource Centre, King Edward School Child Care Centre | Windsor Central |
| Sundowners Day Care and Resource Centre, L A Desmarais School Child Care Centre | Windsor East |
| Sundowners Day Care and Resource Centre, LaSalle Public School Child Care Centre | LaSalle |
| Sundowners Day Care and Resource Centre, Our Lady of Perpetual Help School Child Care Centre | Windsor Central |
| Sundowners Day Care and Resource Centre, Princess Elizabeth School Child Care Centre | Windsor East |
| Sundowners Day Care and Resource Centre, Roseland School Child Care Centre | Windsor South |
| Sundowners Day Care and Resource Centre, St John Vianney School Child Care Centre | Windsor East |
| Sundowners Day Care and Resource Centre, Talbot Trail School Child Care Centre | Windsor South |
| Temple Tots Day Care Centre | London |
| Tilbury Tots Early Learning Centre, Main Site - Tilbury | Tilbury |
| Tilbury Tots Early Learning Centre, T.T.E.L.C. at École Ste-Marie | Chatham |
| Tilbury Tots Early Learning Centre, T.T.E.L.C. at École St-Francis | Tilbury East |
| Tilbury Tots Early Learning Centre, T.T.E.L.C. at École St-Paul | Stoney Point |
| Tilbury Tots Early Learning Centre, T.T.E.L.C. at Merlin Area Public School | Merlin |
| Tilbury Tots Early Learning Centre, T.T.E.L.C. at Pain Court Hub, Sainte-Catherine Garderie | Pain Court |
| Tilbury Tots Early Learning Centre, T.T.E.L.C. at St. Francis Catholic School | Tilbury |
| | Tilbury Tots Early Learning Centre, T.T.E.L.C. at Tilbury Area Public School | Tilbury |
| Tilbury Tots Early Learning Centre, T.T.E.L.C. at Wheatley Area Public School | Wheatley |
| Toy Box Inc (The), Early Childhood Education Centre, Howard Ave Site | Windsor South |
| Toy Box Inc (The), Early Childhood Education Centre, McDougal Site | Windsor South |
| | Wee Watch Enriched Home Child Care, Windsor East | Windsor East |
| | Wee Watch Enriched Home Child Care, Windsor West | LaSalle |
| | Wee Watch Enriched Home Childcare - London | London |
| Western Day Care Centre - Emery Street West | London |
| Western Day Care Centre - Hyde Park | London |
| Wheatley Day Care Centre | Wheatley |
| Whitehills Childcare Association | London |
| Whitehills Childcare Association, School Age Programs | London |
| Whitehills Childcare Association, St Thomas Aquinas Early Childhood Education Centre | London |
| Whitehills Childcare Association, Wilfrid Jury Early Childhood Education Centre and School Age Program | London |
| | Ontario. Ministry of Community and Social Services, Children's Services, Administration Office, Leamington | Leamington (Town of) |
| Ontario. Ministry of Community and Social Services, Children's Services, Child Care Coordination Service, Administration Office, Windsor | Windsor Central |
| YMCA, Chatham-Kent Family YMCA | Chatham |
| YMCA of Chatham-Kent, Our Little Place YMCA Child Development | Chatham |
| YMCA of Western Ontario, Child Care Services | London |
| YMCA of Western Ontario, Child Care Services, Blessed Kateri YMCA Child Care | London |
| YMCA of Western Ontario, Child Care Services, East London YMCA Child Care | London |
| YMCA of Western Ontario, Child Care Services, Kids and Co YMCA Child Care | London |
| YMCA of Western Ontario, Child Care Services, Kidworks YMCA Child Care | London |
| YMCA of Western Ontario, Child Care Services, Lorne Avenue YMCA Child Care | London |
| YMCA of Western Ontario, Child Care Services, Northbrae YMCA Child Care | London |
| YMCA of Western Ontario, Child Care Services, Sir John A MacDonald YMCA Child Care | London |
| YMCA of Western Ontario, Child Care Services, St Marguerite d'Youville YMCA Child Care | London |
| YMCA of Western Ontario, Child Care Services, St Patrick YMCA School Age Program | Lucan |
| YMCA of Western Ontario, Child Care Services, St Theresa YMCA Child Care | London |
| YMCA of Western Ontario, Child Care Services, St Vincent de Paul YMCA Child Care | Strathroy |
| YMCA of Western Ontario, Child Care Services, Strathroy Nursery School | Strathroy |
| YMCA of Western Ontario, Child Care Services, UCC Flexcare Program | London |
| YMCA of Western Ontario, Child Care Services, University Child Care | London |
| YMCA of Western Ontario, Child Care Services, Windy Woods YMCA Child Care | London |
| YMCA of Western Ontario, Child Care Services, YMCA School Age and Extended Day Programs | London |
| YMCA St. Anne Child Care, First Base School Age Daycare | Sarnia |
| Your Wooden Tree House, Child Care Centre, Belle River Site | Belle River (Town of) |
| Your Wooden Tree House, Child Care Centre, Emeryville | Emeryville |
| Youth and Family Resource Network of Essex County, Harrow Branch | Harrow |
| Youth and Family Resource Network of Essex County, Babysitter Safety Course | Kingsville |
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